一种细菌的氨酸酸酶抑制了植物模式识别受体激活
Alberto P Macho1, Benjamin Schwessinger, Vardis Ntoukakis
1The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK.
概括
植物免疫受体,如阿拉比多普西素受体激酶EF-TU受体 (EFR) 被氨酸酸化激活. 一种细菌酸酶抑制了这种作用,突出了控制植物免疫力的分子战斗.
科学领域:
- 植物免疫力 植物免疫力
- 分子信号传递是分子信号传递.
- 细菌病原发生的细菌.
背景情况:
- 天生的免疫包括模式识别受体 (PRRs) 检测病原体相关的分子模式 (PAMPs).
- 许多植物PRR是受体激酶,对于识别微生物入侵者至关重要.
研究的目的:
- 为了研究阿拉比多opsis EF-TU受体 (EFR) 在连接体结合时的激活机制.
- 阐明氨酸酸化在EFR激活和植物免疫反应中的作用.
主要方法:
- 带结合试验用于研究EFR激活.
- 位点定向突变发生,以确定关键的酸化位点.
- 使用Pseudomonas syringae进行植物病原体相互作用的分析.
主要成果:
- 在与elf18结合后,EFR通过对氨酸残留的酸化来激活.
- 铁素836 (Y836) 的酸化对于EFR激活和对Pseudomonas注射器的免疫是必不可少的.
- 细菌氨酸酸酶HopaO1通过减少其酸化来禁用EFR,从而抑制植物免疫力.
结论:
- EFR激活和随后的植物免疫通过氨酸酸化来调节.
- 主体PRR酸化和病原体酸酶之间存在分子竞争,以控制抗菌免疫力.
更多相关视频
11:50Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
Published on: October 1, 2015
21.9K
04:11Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana
Published on: May 21, 2019
12.8K
相关概念视频
Cell Signaling in Plants
4.5K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
4.5K
Riboswitches
8.0K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.0K
Photoreceptors and Plant Responses to Light
22.5K
Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.
22.5K
Amplifying Signals via Enzymatic Cascade
15.3K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
15.3K
Receptor Tyrosine Kinases
15.5K
Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...
15.5K
Defenses Against Pathogens and Herbivores
21.6K
Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.
21.6K
