人类细胞类型和组织中活性增强剂的地图
Robin Andersson1, Claudia Gebhard2, Irene Miguel-Escalada3
1The Bioinformatics Centre, Department of Biology & Biotech Research and Innovation Centre, University of Copenhagen, Ole Maaloes Vej 5, DK-2200 Copenhagen, Denmark.
Nature
|March 28, 2014
概括
研究人员创建了一个人类细胞中活性增强剂的地图. 本资源揭示了增强剂的特性及其在基因调节中的作用,有助于研究细胞分化和疾病.
科学领域:
- 基因组学和分子生物学
- 细胞和发育生物学
背景情况:
- 增强剂是控制多细胞生物中的基因表达的关键调节元素.
- 了解增强剂的功能,活性和点对于破译细胞分化和恒温是必不可少的.
- 以前对增强剂特性及其体内转录活性的知识是有限的.
研究的目的:
- 创建一个全面的活跃的地图,在各种人类组织和细胞类型的体内体内转录增强剂.
- 描述这些增强剂的分子特性和调节活性.
- 为监管计划的比较分析和疾病相关遗传变异的识别提供资源.
主要方法:
- 利用了FANTOM5样本面板,涵盖了广泛的人类组织和细胞类型.
- 分析了增强剂关联的RNA转录,包括它们的方向性,拼接状态和对外体体退化的敏感性.
- 开发了一个在线地图集,集成增强器数据以供公众访问和分析.
主要成果:
- 确定增强剂与CpG贫乏的信使RNA促进剂具有共同特征.
- 证明增强剂产生双向,外体敏感,短,未结合的RNA与增强剂活性直接相关.
- FANTOM5增强剂地图使监管计划的深入比较,疾病相关SNP的识别和增强剂类型的分类成为可能.
结论:
- FANTOM5增强剂地图是研究细胞类型特定和无处不在的增强剂的宝贵资源.
- 增强剂冗余在基因表达强度上起作用,而不是表达模式.
- 这本地图书促进了对基因调节,细胞分化和疾病遗传基础的研究.
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