Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

20.2K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
20.2K
Reporter Genes02:11

Reporter Genes

11.4K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.4K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.4K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.4K
Regulated mRNA Transport02:22

Regulated mRNA Transport

5.7K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
5.7K
What is Gene Expression?01:36

What is Gene Expression?

10.1K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
10.1K
What is Gene Expression?01:42

What is Gene Expression?

132.7K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
132.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Coordinated regulation of mRNA translation and stability by ZC3H7A and ZC3H7B RNA-binding proteins.

Cell reports·2026
Same author

Translational control in the spinal cord regulates gene expression and pain hypersensitivity in the chronic phase of neuropathic pain.

eLife·2026
Same author

Dietary Leucine Requirement and Its Effects on Growth, Immunity, Antioxidant Capacity and Protein Metabolism in Pacific White Shrimp, Penaeus vannamei.

Marine biotechnology (New York, N.Y.)·2026
Same author

SNCA triplication disrupts proteostasis and extracellular architecture prior to neurodegeneration in human midbrain organoids.

NPJ Parkinson's disease·2026
Same author

MechanoMR microparticle (M<sup>3</sup>) sensors reveal dynamic stress loading as a driver of epithelial-mesenchymal transition.

bioRxiv : the preprint server for biology·2025
Same author

TMEM145 is a key stereociliary component in the link structures of outer hair cells and mediates the secretion of stereocilin and tubby.

Nature communications·2025

相关实验视频

Updated: May 1, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

8.2K

通过局部翻译来远程控制基因功能.

Hosung Jung1, Christos G Gkogkas2, Nahum Sonenberg3

  • 1Department of Anatomy, Brain Research Institute, and Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, South Korea.

Cell
|April 1, 2014
PubMed
概括

RNA局部化引导蛋白质合成到特定的细胞部位,这对细胞功能至关重要. 这一过程在神经元中尤为重要,确保了蛋白质的适当局部化和平衡.

更多相关视频

Quantitative Immunofluorescence to Measure Global Localized Translation
09:13

Quantitative Immunofluorescence to Measure Global Localized Translation

Published on: August 22, 2017

9.5K
In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation
09:13

In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation

Published on: April 30, 2014

12.2K

相关实验视频

Last Updated: May 1, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

8.2K
Quantitative Immunofluorescence to Measure Global Localized Translation
09:13

Quantitative Immunofluorescence to Measure Global Localized Translation

Published on: August 22, 2017

9.5K
In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation
09:13

In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation

Published on: April 30, 2014

12.2K

科学领域:

  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学
  • 神经科学是一个神经科学.

背景情况:

  • 蛋白质的功能与其亚细胞位置密切相关.
  • RNA局部化是一种控制蛋白质定位的进化保守机制.
  • 局部mRNA翻译使现场蛋白质合成成为可能,影响细胞信号传递和蛋白质组平衡.

研究的目的:

  • 审查RNA局部化和局部翻译方面的关键发现.
  • 讨论空间基因功能控制的含义.
  • 突出这些机制在神经元区的意义.

主要方法:

  • 审查关于RNA本地化和本地翻译的现有文献.
  • 对证明局部mRNA和蛋白质合成作用的研究进行分析.
  • 讨论实验证据和理论含义.

主要成果:

  • RNA局部化确保蛋白质在特定的亚细胞部位合成.
  • 局部翻译赋予了独特的信号特性,并维持了蛋白质组的平衡.
  • RNA局部化和翻译的失调导致神经元缺陷.

结论:

  • RNA局部化和局部翻译是基因功能空间控制的基础.
  • 这些机制在远端神经元区中尤为重要.
  • 了解这些过程为神经元发育,电线和生存提供了洞察力.