内体是细菌感应和NOD2信号发送的专门平台
Norihiro Nakamura1, Jennie R Lill1, Qui Phung1
1Genentech, 1 DNA Way, South San Francisco, California 94080, USA.
Nature
|April 4, 2014
概括
主体免疫细胞使用托尔类受体 (TLR) 和NOD类受体 (NLR) 来检测病原体. 这项研究揭示了载体SLC15A3和SLC15A4对于内体内细菌成分的NOD2受体激活至关重要.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 微生物病原体的宿主检测依赖于托尔类受体 (TLR) 和NOD类受体 (NLR).
- 像NOD2一样,NLRs感知细胞质微生物产物,但结合体从内分体中脱离结合体的机制尚不清楚.
- NOD2变体与炎症性肠病和其他慢性炎症状况有关.
研究的目的:
- 为了研究NOD2连接物离开内分泌体的机制.
- 为了确定参与感知内性衍生细菌成分的宿主因素.
- 阐明树突细胞内体在病原体感应中的作用.
主要方法:
- 研究了内溶体载体SLC15A3和SLC15A4.4的表达和功能.
- 利用树突细胞和TLR刺激模型.
- 评估了通过muramyl dipeptide (MDP) 激活NOD2的载体的要求.
主要成果:
- SLC15A3和SLC15A4主要由树突细胞表达,特别是在TLR刺激后.
- 这些载体调解了细菌衍生成分,包括NOD2配体MDP,从内分泌体的退出.
- 增强的输送体表达导致内体膜管体的形成,促进NOD2依赖的反应.
结论:
- SLC15A3和SLC15A4对于内体衍生细菌成分的NOD2感应至关重要.
- 树突细胞内体作为专业的平台,用于光线和细胞质的病原体传感.
- 这突出了先天性免疫识别的新途径及其在炎症性疾病中的含义.
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