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对于梅克尔细胞机械传导,需要Piezo2

Seung-Hyun Woo¹, Sanjeev Ranade¹, Andy D Weyer²

¹Howard Hughes Medical Institute, Molecular and Cellular Neuroscience, The Scripps Research Institute, La Jolla, California 92037, USA.

|April 11, 2014

概括

默克尔细胞使用Piezo2通道来感知温柔的触摸. 这一发现揭示了Piezo2的存在.

科学领域:

神经科学是一个神经科学.
机械生物学机械生物学
身体上的感觉身体上的感觉

背景情况:

默克尔细胞-神经元复合体对于温和的触摸感觉至关重要,但其机械转导机制仍然未知.
在感知机械力方面,默克尔细胞和 afferent 神经纤维的作用受到争论.
了解触觉的分子基础是神经科学的一个基本挑战.

研究的目的:

为了阐明默克尔细胞中机械转导的分子机制.
确定Piezo2通道在轻微触摸感觉中的作用.
研究默克尔细胞对默克尔细胞神经元复合体的功能贡献.

主要方法:

在体外电生理学以记录默克尔细胞中的触摸敏感电流.
基因改造小鼠的生成,具有皮肤的Piezo2缺乏,特别是皮肤的默克尔细胞.
感觉神经反应的体内电生理学记录和触摸敏感性的行为测试.

主要成果:

默克尔细胞表现出依赖于Piezo2表达的触摸敏感电流.
在默克尔细胞中缺乏Piezo2的小鼠显示缓慢适应反应减少和轻柔触摸行为受损.
有证据支持一种两种受体位点模型,包括梅克尔细胞和附带纤维.

结论:

Piezo2被确定为默克尔细胞中的主要机械传导通道,用于轻柔的触摸.
这项研究提供了第一个证据,证明了皮埃佐通道在哺乳动物机械感觉中的生理作用.
一个双受体模型突出了默克尔细胞和关联神经元的合作感应功能.

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