cdc2的可逆氨酸酸化:脱酸化伴随着在进入线粒分裂过程中的激活
A O Morla1, G Draetta, D Beach
1Department of Biology, University of California, La Jolla, San Diego 92093.
Cell
|July 14, 1989
概括
细胞循环调节涉及cdc2激酶的氨酸酸化. 脱化cdc2对于线粒激活至关重要,突出其在细胞周期进展中的作用.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 在细胞周期期间,Cdc2激酶活性受到严格调节.
- 氨酸酸化在调节cdc2活性方面发挥着作用.
研究的目的:
- 为了研究cdc2的氨酸酸化和脱酸化在小鼠3T3纤维细胞的细胞周期中的作用.
- 为了确定cdc2酸化状态与其酶活性之间的关系.
主要方法:
- 在不同细胞周期阶段对cdc2氨酸酸化水平的分析.
- 测定cdc2.2.的组织素H1激酶活性.
- 使用酸酶抑制剂抑制铁酸脱化.
主要成果:
- 在G1到G2阶段,cdc2的氨酸酸化会增加,而在线粒分裂过程中会减少.
- 氨酸酸化的cdc2作为一个基因素H1激酶是不活跃的.
- 氨酸脱酸化的抑制导致G2细胞循环停止.
结论:
- 氨酸脱化cdc2是其在线粒分裂期间激活的重要一步.
- Cdc2调节涉及整个细胞循环中铁酸化状态的动态变化.
相关概念视频
01:45Positive Regulator Molecules
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
02:39Positive Regulator Molecules
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...