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光子晶体激酶生物传感器

Kelsey I MacConaghy1, Christopher I Geary, Joel L Kaar

  • 1Department of Chemical and Biological Engineering, University of Colorado , Boulder, Colorado 80309, United States.

Journal of the American Chemical Society
|April 26, 2014
PubMed
概括

我们创建了一个新的生物传感器,使用一种特殊的水凝来检测酶活性. 这种无标签的方法追踪光衍射的变化,以测量酶酸化,提供了一种新的选方法.

科学领域:

  • 生物技术是生物技术.
  • 材料科学 材料科学 材料科学
  • 生物化学 生物化学

背景情况:

  • 激酶活性在细胞信号传递和疾病中至关重要.
  • 目前用于酶活性查的方法通常需要标签,并且可能是复杂的.
  • 需要开发敏感的,无标签的检测方法.

研究的目的:

  • 开发一种新的,无标签的生物传感器,用于检测酶活性.
  • 为了利用响应性聚合物凝用于光学读取酶介导酸化.
  • 建立一种使用光学变化量化酸化程度的方法.

主要方法:

  • 聚合物水凝的制造,其中包含一个结晶的合体阵列.
  • 水凝的设计在酸化后可逆膨胀.
  • 监测光学衍射的变化作为酶活性的读数.
  • 使用衍射测量和电荷染色进行确认.

主要成果:

  • 开发的水凝在对酸酸化的反应中显示出可逆性胀.
  • 激酶治疗诱导了水凝光学衍射的时间和剂量依赖的变化.
  • 离子聚合物网络的理论模型准确地描述了观察到的光学响应.

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  • 该系统允许在没有标签的情况下量化酸化.

    • 结论:

    • 一种新型的酶响应的聚合物凝生物传感器已经成功开发出来.
    • 生物传感器可以通过酸化诱导的胀来无标签,光学检测激酶活性.
    • 该系统提供了一种敏感和可量化的方法来选激酶活性.