单细胞基因组学揭示了野生Prochlorococcus中数百个共存的亚种群
Nadav Kashtan1, Sara E Roggensack, Sébastien Rodrigue
1Department of Civil and Environmental Engineering, Massachusetts Institute of Technology (MIT), 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
概括
海洋蓝藻菌 Prochlorococcus 种群包含数百个不同的基因组亚种群. 这些古老的亚种群表明在野外存在稳定的利基分区,揭示了广泛的微生物多样性.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 海洋生物学 海洋生物学
背景情况:
- 微生物物种表现出广泛的基因组多样性,经常通过培养分离物和转基因组学进行研究.
- 野生,未培养微生物种群的细胞对细胞基因组合在很大程度上仍然没有被描述.
研究的目的:
- 用大规模单细胞基因组学研究野生,未培养的Prochlorococcus种群的基因组组成.
- 了解全球丰富的海洋蓝藻细菌中的基因组多样性和种群结构的程度.
主要方法:
- 大规模单细胞基因组学技术的应用.
- 对个别Prochlorococcus细胞中的基因组骨干和灵活基因组的分析.
主要成果:
- 菌种群由数百个子种群组成,每个子种群都有独特的基因组骨干.
- 基因组骨干包括与特定的灵活基因组相关的独特核心基因等位基因.
- 据估计,这些子群体在数百万年前已经分离,这表明古代的利基划分.
结论:
- 野生Prochlorococcus种群表现出具有古老起源的显著基因组亚体结构.
- 稳定的利基划分可能推动了众多亚种群的共存.
- 通过共存的亚种群的广泛的基因组多样性可能是混合环境中大型,自由生活的细菌群体的共同特征.
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