从乌的光受体释放出内源刺激性氨基酸
1Department of Ophthalmology, University of California, San Francisco 94143-0730.
Nature
|October 12, 1989
概括
研究人员检测到,触发性氨基酸 (EAA) 从的光受体中释放出来. 这提供了强有力的证据,即EAA是棒和的神经递质,影响视觉信号.
科学领域:
- 神经科学是一个神经科学.
- 视网膜生理学 视网膜生理学
- 神经传递的神经传递
背景情况:
- *视觉信息传输依赖于光受体和第二阶神经元之间的化学突触.
- *刺激性氨基酸 (EAA) 是假设的神经递质,得到了对视网膜神经元的药理学研究的支持.
- * 缺乏从光受体释放内源EAA的直接证明.
研究的目的:
- *为从光受体释放内源激发性氨基酸 (EAA) 提供直接证据.
- * 为了确定视网膜光受体使用的EAA神经递质的特定类型.
主要方法:
- *使用了来自老鼠海马神经元的外外膜补丁.
- * 检查了隔离的乌光受体及其突触终端.
- * 应用电刺激和化对光感受器.
- *在切除的膜贴片中监测道活动.
主要成果:
- *电刺激和化增加了膜贴片中的50pS通道的开放频率.
- *这些通道是谷氨酸激活通道的特征,也对阿斯巴甜酸和NMDA有反应.
- *在几个光受体-补丁对中,在突触终端附近观察到自发的通道活动.
结论:
- * 证明EAA从隔离的乌光受体释放出来.
- * 提供了强有力的证据,支持EAA在杆状和状细胞中充当神经递质的假设.
- *鉴定了谷氨酸作为海视网膜中可能的EAA神经递质.
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