pT181等离子体复制受反转录驱动的转录减弱器调节.
R P Novick1, S Iordanescu, S J Projan
1Public Health Research Institute, New York, New York 10016.
Cell
|October 20, 1989
概括
像PT181这样的葡萄球菌等离子体使用反感性RNA来调节复制. 这些反转录引发了早产的mRNA终结,控制了启动蛋白的表达.
科学领域:
- 分子生物学分子生物学
- 微生物学 微生物学
- 遗传学 是一个遗传学.
背景情况:
- 葡萄球菌等离子体利用反意义RNA进行复制控制.
- 反意义RNAs (反转录) 阻断了启动蛋白的表达.
- pT181是研究反意义RNA介导调节的模型等离子体.
研究的目的:
- 阐明 pT181 反转录调节启动蛋白表达的机制.
- 研究mRNA二次结构在反意义RNA介导基因调节中的作用.
- 为了区分pT181减弱系统与传统的基于核糖体延迟的减弱器.
主要方法:
- 对mRNA二次结构形成的分析.
- 调查反转录和目标mRNA之间的相互作用.
- 将反意义RNA驱动的衰减与tRNA诱导的衰减进行比较.
主要成果:
- pT181的反转录会导致mRNA的过早终止 (减弱).
- 这种终结是通过在启动器开始密码子附近促进毛结构来实现的.
- 一个上游的"先驱"序列通常通过与终结器头发针相互作用来阻止终止.
结论:
- 在pT181等离子体中,用于复制控制,采用一种基于RNA的新型反感应衰减机制.
- 这种系统从根本上不同于传统的衰减器,这些衰减器依赖于核糖体延迟.
- 反感性RNA为细菌等离子体中调节基因表达提供了一种独特的策略.
相关概念视频
01:43Proofreading
52.0K
Overview
52.0K
02:10Translesion DNA Polymerases
9.3K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
9.3K
02:37Restarting Stalled Replication Forks
5.1K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.1K
02:46Genome Copying Errors
4.3K
DNA replication is a well-evolved process that copies millions of base pairs with high fidelity during each cell division. Occasionally a wrong base or a long stretch of wrong bases may get added to the daughter strands. If the errors are left unchecked, cells might accumulate several mutations that might endanger their survival. Therefore, the copying errors are checked and repaired at three levels.
4.3K