一个半合成的有机体,具有扩展的遗传字母
Denis A Malyshev1, Kirandeep Dhami1, Thomas Lavergne1
1Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Nature
|May 9, 2014
概括
研究人员创造了第一个稳定传播扩展遗传字母的细菌,使用非自然基对 (UBPs). 这一突破克服了在生物体内进口和复制合成DNA的挑战.
科学领域:
- 合成生物学 合成生物学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 有机体使用双基对遗传字母 (A-T,G-C).
- 非自然基对 (UBPs) 在体外已经被创造出来,但细胞融合仍然是一个挑战.
- 扩大遗传字母需要细胞吸收非天然的核酸三酸盐和忠实复制.
研究的目的:
- 为了设计一种能够稳定繁殖扩大基因字母的细菌.
- 证明内生细胞机器对非自然三酸盐的进口和利用.
- 评估生物体内的非自然基因对的稳定性和生物影响.
主要方法:
- 从疏水核基开发出非自然的基对 (UBP),以d5SICS-dNaM为例.
- 改造了大肠杆菌以表达一种藻类核酸三酸转运体,以进口d5SICSTP和dNaMTP.
- 使用内源复制机器复制含有d5SICS-dNaM UBP的等离子体.
主要成果:
- 在大肠杆菌中实现了d5SICSTP和dNaMTP的有效导入.
- 内源聚合酶准确地复制了含有d5SICS-dNaM UBP.的等离子体DNA.
- 没有观察到任何显著的生长负担,并且UBP没有通过DNA修复途径有效地切除.
结论:
- 成功创造了第一个稳定传播扩展遗传字母的有机体.
- 证明了在活体细菌系统中使用非自然基对的可行性.
- 这项工作为开发新的生物功能和应用开辟了道路.
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