在动态的超分子宿主之间进行细胞内客体交换
Subramani Swaminathan1, Colin Fowley, Bridgeen McCaughan
1Laboratory for Molecular Photonics, Department of Chemistry, University of Miami , 1301 Memorial Drive, Coral Gables, Florida 33146-0431, United States.
Journal of the American Chemical Society
|May 13, 2014
概括
两性聚合物自组装成纳米粒子,有效地捕获和转移分子捐赠者和接受者之间的能量. 这些动态纳米载体可以在癌细胞内输送和同定位货物,以进行向的细胞内能量传输.
科学领域:
- 聚合物化学 聚合物化学
- 纳米技术纳米技术
- 生物医学工程 生物医学工程
背景情况:
- 两性聚合物对于开发新型药物输送系统至关重要.
- 自组装纳米粒子为分子相互作用提供受控环境.
- 高效的细胞内输送和货物联合定位仍然是纳米医学中的挑战.
研究的目的:
- 合成和描述能够形成能传输能量纳米粒子的两性聚合物.
- 研究这些纳米粒子的货物载荷,能量传输效率和动态交换特性.
- 评估这些纳米载体在癌细胞中进行细胞内传递和向能量转移的潜力.
主要方法:
- 聚甲酸脊柱与基和基乙烯糖醇链的合成.
- 纳米粒子在水性缓冲体中的自组合和水力动力直径的表征.
- 在纳米粒子疏水核中装载 antracen 捐赠体和 borondipyrromethene 接受体.
- 福斯特共振能量转移 (FRET) 的效率测量.
- 细胞培养研究涉及宫癌细胞和纳米粒子化,用于细胞内输送和能量转移评估.
主要成果:
- 两性聚合物自组装成15纳米纳米粒子,其疏水性与疏水性比为2.5.
- 纳米粒子在封装的捐赠者和接受者之间实现了95%的激发能量传输效率.
- 超分子组件在水性介质中展示了快速的客体交换,使染色体的同定位成为可能.
- 纳米粒子成功地穿过了宫癌细胞的膜,促进了细胞内部的同位化和能量转移.
- 顺序化策略允许货物交换和能量转移仅在细胞内环境内.
结论:
- 动态的两性聚合物纳米颗粒可以有效地封装和共同定位分子客.
- 这些纳米载体通过使细胞环境内的货物交换成为可能,促进了有针对性的细胞内能量传输.
- 能够独立地传递和触发细胞内的物种相互作用的能力为theranostics开辟了新的途径.
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