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相关概念视频

RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

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Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
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Eukaryotic RNA Polymerases00:58

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RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
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相关实验视频

Updated: Apr 29, 2026

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
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快照:核RNAPII转录修改核RNAPII转录修改

Manfred Schmid1, Torben Heick Jensen1

  • 1Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, Denmark.

Cell
|May 27, 2014
PubMed
概括
此摘要是机器生成的。

RNA聚合酶II产生各种RNA分子,如mRNA和miRNA. 这些关键的转录在细胞核中经历了重要的修改和处理,由特定的酶促进.

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Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
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相关实验视频

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Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
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Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物化学 生物化学

背景情况:

  • RNA聚合酶II是基因表达的核心,产生不同的RNA类型.
  • 新生的RNA转录需要广泛的转录后修改.
  • 核酶在RNA处理和成熟中起着关键作用.

研究的目的:

  • 提供RNA处理途径的简要概述.
  • 要突出涉及核RNA修饰的酶.
  • 为了说明由RNA聚合酶II生成的RNA转录的多样性.

主要方法:

  • 文献综述和当前研究的综合.
  • RNA处理步骤的视觉表示 (快照格式).
  • 专注于关键酶及其功能.

主要成果:

  • 详细描述了mRNA,miRNA,lncRNA和sn(o) RNA的处理过程.
  • 确定核RNA修饰所必需的酶.
  • 澄清RNA成熟过程中的序列步骤.

结论:

  • RNA聚合酶II转录是复杂的RNA处理级联中的第一步.
  • 核酶对于生成功能性RNA分子是不可或缺的.
  • 了解这些过程对于理解基因调节和细胞功能至关重要.