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相关概念视频

Proteomics01:33

Proteomics

7.5K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Protein Networks02:26

Protein Networks

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An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
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Protein Networks02:26

Protein Networks

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Protein Organization01:24

Protein Organization

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Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence....
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Protein Organization01:24

Protein Organization

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Protein Organization01:13

Protein Organization

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Overview
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Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
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人类蛋白质组的地图草案.

Min-Sik Kim1, Sneha M Pinto2, Derese Getnet3

  • 11] McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA [2] Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

Nature
|May 30, 2014
PubMed
概括

研究人员使用质谱学创建了一个人类蛋白质组图,识别了来自17,294个基因的蛋白质. 这份全面的蛋白质地图有助于生物医学研究的健康和疾病.

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科学领域:

  • 蛋白质组学是指蛋白质组学.
  • 基因组学就是基因组学.
  • 生物医学研究生物医学研究

背景情况:

  • 人类基因组序列可用,但缺乏具有直接蛋白质测量的全面的人类蛋白质组图.
  • 这种差距阻碍了对健康和疾病中的生物过程的全面理解.

研究的目的:

  • 为了创建人类蛋白质组的草稿地图.
  • 使用先进的蛋白质基因技术识别蛋白质和新型蛋白质编码区域.

主要方法:

  • 使用高分辨率的里埃变换质谱法进行了深入的蛋白质原子分析.
  • 分析包括30个组织学上正常的人体样本,跨越各种组织类型和细胞群.
  • 使用了一种蛋白质基因组分析策略.

主要成果:

  • 鉴定了由17,294个基因编码的蛋白质,约占注释的蛋白质编码基因的84%.
  • 发现了新的蛋白质编码区域,包括翻译的伪基因,非编码RNA和上游开放的阅读框架.
  • 开发一个全面的人类蛋白质组目录,作为网络资源提供.

结论:

  • 开发的人类蛋白质组图显著提高了我们对人类生物学的理解.
  • 该资源补充了现有的基因组和转录基因组数据.
  • 预计它将加速生物医学研究和疾病研究.