miR-34/449 miRNAs 需要通过抑制cp110来进行运动性纤维生成
Rui Song1, Peter Walentek2, Nicole Sponer1
11] Division of Cellular and Developmental Biology, MCB Department, University of California at Berkeley, Berkeley, California 94705, USA [2].
Nature
|June 6, 2014
概括
微RNAs miR-34/449 对于运动性纤维生成至关重要,对于呼吸和生殖功能至关重要. 它们的缺乏会导致毛形成和粘膜干净的严重缺陷.
科学领域:
- 分子生物学分子生物学
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
背景情况:
- miR-34/449家族包括六个同源的微RNA (miRNAs),位于三个基因组位置.
- 这些miRNAs主要表达在多状上皮质中,这表明它们在纤维生成中起着关键作用.
研究的目的:
- 调查miR-34/449家族在纤毛发育中的功能及其生理后果.
- 阐明miR-34/449调节乳毛形成的分子机制.
主要方法:
- 产生和分析所有miR-34/449miRNAs缺乏的小鼠.
- 从小鼠和Xenopus模型中评估多细胞 (MCC) 中的状细胞结构和功能.
- 调查中心蛋白Cp110在miR-34/449介导的纤维生成中的作用.
主要成果:
- 缺乏miR-34/449的小鼠表现出产后死亡率,不孕症和呼吸功能障碍,这是由于粘膜干净的受损造成的.
- 缺乏miR-34/449的MCC显示小毛的长度和数量减少,这与基底体成熟和顶端对接的缺陷有关.
- miR-34/449 调节纤毛发育,部分通过转录后抑制Cp110,一种抑制纤毛组合的抑制剂.
- 在缺乏miR-34/449的MCC中,Cp110的敲除挽救了纤维生成.
结论:
- miR-34/449 miRNA家族在调节移动性纤维生成方面发挥着关键的保存作用.
- miR-34/449的失调会导致严重的发育和生理缺陷,与功能受损有关.
- Cp110是一个关键的下游目标,它调解了miR-34/449对基底体成熟和乳毛组合的影响.
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