这种HP1同类犀牛定了一个核复合体,该复合体抑制了piRNA前体拼接
Zhao Zhang1, Jie Wang2, Nadine Schultz3
1Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA; Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester MA 01605, USA; RNA Therapeutics Institute, University of Massachusetts Medical School, 368 Plantation Street, Worcester MA 01605, USA.
Cell
|June 7, 2014
概括
德洛索菲拉犀牛蛋白质定了一个复合物,阻止piRNA前体的拼接,这对于生殖基因组对转子体的防御至关重要. 这种机制将piRNA前体与信使RNA区分开来.
科学领域:
- 表观遗传学和基因调控
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 在生殖线发育过程中,piRNAs对于沉默可移植元素至关重要,形成了适应性基因组防御系统.
- 德洛索菲拉HP1同类物Rhino是生殖线piRNA生产所需的关键蛋白质.
研究的目的:
- 为了阐明犀牛促进生殖线piRNA生产的机制.
- 研究犀牛在调节piRNA前体转录及其拼接中的作用.
主要方法:
- 染色体结合测试以确定犀牛的基因组标.
- 与其他piRNA通路蛋白进行局部化研究 (Cuff, UAP56).
- RNA测序以分析在野生类型和突变条件下的转录拼接和表达.
- 报告员转基因测试评估犀牛在拼接和piRNA生成中的功能.
主要成果:
- 犀牛特别结合于负责piRNA前体生成的异色色团,与piRNA输出直接相关.
- 犀牛,袖子和UAP56在生殖线核焦点中同位化,对于抑制转录拼接至关重要.
- 犀牛,袖口或uap56的突变导致拼接集群转录的增加>100倍.
- 犀牛结合活性足以抑制记者转基因拼接并诱导piRNA生产.
结论:
- 犀牛作为支架,定一个核复合体,抑制piRNA前体转录的拼接.
- 拟议的机制表明,集群转录的停滞拼接是区分piRNA前体和mRNAs的一个关键步骤.
- 这一发现为管理piRNA生物发生和基因组防御在生殖系中的调控机制提供了新的见解.
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