甲基α-D-mannopyranoside的分子识别通过防剂 (糖蛋白) 蛋白
Sen Wang1, Xin Wen, Arthur L DeVries
1Molecular Imaging Program, Stanford University , Stanford, California 94305, United States.
Journal of the American Chemical Society
|June 12, 2014
概括
抗蛋白和糖蛋白 (AF(G) 可以控制甲基α-D-曼诺酸 (MDM) 糖晶的生长. 这一发现为AF(G) P相互作用和潜在的工业应用提供了新的见解.
科学领域:
- 生物化学和材料科学 材料科学
- 探索蛋白质和晶体结构之间的分子相互作用.
背景情况:
- 抗蛋白和糖蛋白 (AF(G) 已知通过结合特定的冰面来抑制冰晶的生长.
- AF(G) P与冰晶面具有显著的结构兼容性.
研究的目的:
- 研究AF(G) Ps在控制甲基α-D-mannopyranoside (MDM) 晶体生长中的潜力,这是一个代表性的pyranose糖.
- 探索AF(G) P和MDM晶体生长面之间的结构兼容性.
主要方法:
- 选定的鱼类AFGP和甲虫AFP具有不同的抗活性作为添加剂.
- 在AF的存在下研究了MDM晶体的生长,使用单晶X射线衍射和极化显微镜等技术.
- 分析了MDM核和计算了关键的自由能量变化.
主要成果:
- AF(G) Ps有效地抑制MDM晶体的生长和再结晶,其有效性与抗活性相关.
- 用AF(G) 培养的MDM晶体显示出更小的尺寸,更好的形状和更高的质量.
- 没有发现新的MDM多态,但核化过程受到AF(G) Ps的影响.
结论:
- AF(G) P与MDM晶体面具有结构兼容性,类似于它们与冰的相互作用.
- MDM作为一种潜在的非冰状晶体模型,用于研究AF(G) P-晶体相互作用.
- 研究结果表明,AF(G) P在糖,食品,制药和材料行业的潜在应用.
相关概念视频
01:24Oligosaccharide Assembly
2.6K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
2.6K
01:14Glycocalyx and its Functions
9.2K
The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
9.2K