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Visualization of Proprioceptors in Drosophila Larvae and Pupae
Published on: June 13, 2012
在Drosophila胚胎发生过程中PS整合素的功能
Cell
|February 10, 1989
概括
脊椎动物纤维菌素受体的同源体,是胚胎发育的关键. 肌球基因中的突变破坏了β整合素子单元的表达,导致胚胎死亡和细胞粘附缺陷.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 分子遗传学 分子遗传学
背景情况:
- 果的位置特异性 (PS) 抗原与脊椎动物整合素,细胞表面受体的一种家族共享同质性.
- 整合素在细胞粘附,迁移和跨物种组织发育中起着至关重要的作用.
- 大虫中的1球基因对于胚胎形态发生是必不可少的.
研究的目的:
- 为了研究虫胚胎发生过程中l(1) 肌球基因产物的功能.
- 确定多菌整合素在胚胎发育和组织组装中的作用.
- 关于PS抗原表达的l(1) 菌球突变的表型的特征.
主要方法:
- 对1的基因分析1) 肌球突变.
- 在野生类型和突变胚胎中免疫学检测PS抗原子单元 (α和β).
- 在胚胎发育过程中对PS抗原定位的分析.
主要成果:
- 1) 肌球突变体缺乏PS抗原的β子单元.
- 缺少β子单元会阻止PSα子单元的细胞表面表达.
- 在野生类型胚胎中,PS抗原定位在中皮-外皮接口和肌肉附着部位.
结论:
- 这一l(1) myospheroid基因编码了Drosophila integrins的β子单元.
- 在胚胎发生过程中,多索菲拉整合素对于中皮和外皮之间的细胞粘附至关重要.
- 细胞外基质和肌肉附着的正确组装取决于功能性的Drosophila整合素.
相关概念视频
01:10Integrins
Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
01:15Activation of Integrins
Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding events provide an effective stimulus.
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding events provide an effective stimulus.