在免疫球蛋白增强剂结合,无子细胞,MyoD和myc蛋白质中发现了一种新的DNA结合和二元化动机
C Murre1, P S McCaw, D Baltimore
1Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142.
Cell
|March 10, 1989
概括
研究人员确定了两种新型cDNA,它们可以结合DNA. 它们的二分化和DNA结合能力至关重要,涉及一种类似于已知的发育基因的螺旋-循环-螺旋模式.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 蛋白质-DNA相互作用
背景情况:
- 免疫球蛋白卡帕链增强剂含有特定的DNA序列 (kappa E2),由调节性蛋白质识别.
- 现有的知识将某些基因家族 (例如,Drosophila achaete-scute,twist,myc) 与发育过程和DNA结合联系起来.
研究的目的:
- 识别和描述参与调节免疫球蛋白卡帕链增强剂的新型cDNA.
- 研究已识别的cDNA的结构和功能性质,特别是它们的二分化和DNA结合机制.
主要方法:
- 隔离和克隆两个特定的cDNAs.
- 对已知发育基因的序列同质性分析 (Drosophila没有女儿,myc,MyoD,achaete-scute,twist).
- 同类区域的结构预测,重点是两螺旋和保存的疏水残留物.
- 实验验证识别的基因在二分化和DNA结合中的作用.
主要成果:
- 两种cDNA被分离出来,它们的二元化产物特别与kappa E2 DNA序列结合.
- 这些cDNA包含一个同源区域,与Drosophila无子和其他发育基因家族有相似之处.
- 这个同质区域表现出一种潜在的螺旋环螺旋结构,具有高度保存的疏水性残留物,对于二分化和DNA结合至关重要.
结论:
- 一个新型的动机,在物种和基因家族中保存,对于蛋白质二分化和特定的DNA结合至关重要.
- 这些发现为调节免疫球蛋白基因表达和更广泛的发育途径的分子机制提供了新的见解.
相关概念视频
02:32Histone Modification
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
02:13Cooperative Binding of Transcription Regulators
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
02:23Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
02:13Cooperative Binding of Transcription Regulators
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
02:23Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
02:32Histone Modification
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...