氨酸重复和相邻的DNA结合域介导功能cFos-cJun异构体的形成
1Howard Hughes Medical Institute, Department of Biochemistry, University of California, Berkeley 94720.
Science (New York, N.Y.)
|March 31, 1989
概括
俊综合体将癌症与基因调节联系在一起. 和蛋白形成异构体,使得DNA结合并推进对原型基因功能的理解.
科学领域:
- 分子生物学分子生物学
- 瘤发生的发生因子.
- 转录条例 转录条例 转录条例
背景情况:
- 增强剂结合因子的AP-1家族对于将瘤发生与转录调节联系起来至关重要.
- 细胞Fos (cFos) 和细胞Jun (cJun) 蛋白质构成AP-1家族中的一个关键复合体.
研究的目的:
- 研究Fos和Jun蛋白质的蛋白质-蛋白质相互作用和DNA结合特性.
- 阐明了cFos-cJun复合体的形成和功能背后的分子机制.
主要方法:
- 在cFos和cJun蛋白质的体外合成和共翻译.
- 对同质和异质复合物的DNA结合能力的分析.
- 针对cFos的突变发生,以确定蛋白质相互作用和DNA结合的关键域.
主要成果:
- 同位体cJun结合AP-1位点,但cFos没有.
- 同转化cFos和cJun形成一个稳定的异构体,具有AP-1/cJun的DNA结合特性.
- 俊B和vJun也与cFos.形成了DNA结合复合体.
- 在cFos中的leucine重复对于绑定cJun至关重要,与leucine拉链模型一致.
- 对于cFos-cJun异构体中DNA结合而言,需要在cFos中的一个新域,与白重复区分开来.
结论:
- 氨酸重复介导异质蛋白质复合体的形成.
- 实验证据支持白重复在蛋白质与蛋白质相互作用中的作用.
- 这项研究增强了对涉及Fos和Jun等原型瘤基因产品的分子机制的理解.
相关概念视频
02:36RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
02:04Co-activators and Co-repressors
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
02:23Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
02:36RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
02:04Co-activators and Co-repressors
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
02:23Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...