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在核酸中测量生理温度距离,使用基于三甲基的旋转标签和脉冲双极EPR光谱仪

Georgiy Yu Shevelev¹, Olesya A Krumkacheva, Alexander A Lomzov

¹Institute of Chemical Biology and Fundamental Medicine, §International Tomography Center, and #N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch of the Russian Academy of the Sciences (SB RAS) , Novosibirsk 630090, Russia.

Journal of the American Chemical Society

|June 26, 2014

概括

研究人员使用电子磁共振 (EPR) 在体温下测量了DNA中的纳米尺度距离. 这一突破使得在自然,生理条件下研究核酸结构成为可能.

科学领域:

生物物理学的生物物理.
结构生物学结构生物学
生物化学生物化学

背景情况:

在生理条件下确定纳米级生物分子结构是很困难的.
电子磁共振 (EPR) 是一种用于探测分子结构的强大技术.

研究的目的:

开发和演示一种在生理温度下测量核酸中长距离的方法.
建立电子磁共振 (EPR) 作为研究溶液中的生物分子结构的可行工具.

主要方法:

带有三甲基的10米尔DNA复合体的现场定向旋转标记.
在水溶液中的吸附剂上固定标记的DNA.
在310K (37°C) 处使用双量子相干EPR光谱学的研究.

主要成果:

成功开发了最佳的三甲基基标签.
建立一个高效的现场定向的旋转标签和固定协议.
在生理温度下精确测量DNA中大约4.6nm的距离.

结论:

本研究介绍了使用EPR.在生理温度下的核酸中的第一个距离测量.
开发的方法允许在接近本地条件下对生物分子进行高精度的结构分析.
这种方法为研究生物相关环境中的核酸动态和相互作用开辟了新的途径.

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