中心体,而不是核,在Drosophila胚胎中启动极细胞的形成
1Department of Biochemistry, Imperial College of Science and Technology, London, England.
Cell
|May 19, 1989
概括
阿菲迪科林阻止了Drosophila胚胎中的DNA合成,但中心体仍然迁移并组织细胞骨. 单独的中间体可以启动极细胞的形成,这表明它们在早期发育中的关键作用.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 早期的胚胎发育依赖于协调的核和中心体行为.
- 中心体在组织微管和细胞分裂方面发挥着至关重要的作用.
研究的目的:
- 为了研究中心细胞在核迁移和极细胞形成中的作用,在Drosophila胚胎中.
- 为了确定中心体是否可以影响细胞骨组织独立于细胞核.
主要方法:
- 在早期的Drosophila胚胎中注射aphidicolin,一种DNA合成抑制剂.
- 使用显微镜观察核和中心体行为,迁移和皮质重组.
主要成果:
- 阿菲迪科林抑制了DNA合成和核分裂,但没有抑制中枢细胞复制或线粒循环.
- 中心细胞向皮层的迁移正常发生,随后发生了管素,活性蛋白和血膜的重组.
- 在后极的中心体中,即使没有核,也会启动极细胞的形成.
结论:
- 单独的中间体可以在到达胚胎表面时指导显著的皮质细胞骨重组.
- 协调的核迁移到皮层很可能是由作用于中心体的力量介导的,而不是核.
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