在Arabidopsis thaliana的初级细胞壁中的水-多糖相互作用来自极化转移固态NMR的极化转移
Paul B White1, Tuo Wang, Yong Bum Park
1Department of Chemistry and Ames Laboratory, Iowa State University , Ames, Iowa 50011, United States.
Journal of the American Chemical Society
|July 2, 2014
概括
植物细胞壁根据多糖相互作用以不同的方式结合水. 固态核磁共振显示,点与水的相互作用比纤维素与水的相互作用更快,影响了水的流动性和主要壁的分布.
科学领域:
- 植物生物学 植物生物学
- 生物物理学的生物物理.
- 材料科学 材料科学 材料科学
背景情况:
- 植物细胞壁是水合的,但水和多糖之间的分子相互作用仍然不清楚.
- 了解这些相互作用对于植物细胞壁结构和功能至关重要.
研究的目的:
- 调查Arabidopsis thaliana中相对于主壁多糖的水接近性和流动性.
- 阐明特定多糖和离子在水-多糖相互作用中的作用.
主要方法:
- 利用极化转移固态核磁共振 (NMR) 技术.
- 转移水质子 (1H) 极化到多糖碳-13 (13C) 信号.
- 分析完整和提取的细胞壁样本,以研究旋转扩散动力学.
主要成果:
- 水-pectin极化转移明显比水-纤维素转移快得多.
- 离子去除和homogalacturonan (HG) 提取最初减缓了旋转扩散,表明水结合的变化.
- 进一步的矩阵多糖提取恢复了旋转扩散率,这表明暴露于水的表面面积增加了.
结论:
- 离子和HG凝会影响结合的水,促进旋转扩散.
- 的去除导致更动态的水,减缓旋转扩散.
- 更快的水 - pectin 旋转扩散支持单个网络模型的植物主要墙壁.
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