一个核酸盐脱酶复合体对于生成乙-CoA和基因素乙化产生很重要
Gopinath Sutendra1, Adam Kinnaird1, Peter Dromparis1
1Department of Medicine, University of Alberta, Edmonton, AB T6G 2B7, Canada.
Cell
|July 5, 2014
概括
线粒体酸盐脱酶复合体 (PDC) 移动到核中,产生乙-CoA用于基因素乙化,并调节细胞循环的进展. 这种核 PDC 推动了表观遗传调节.
科学领域:
- 细胞生物学 细胞生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 生物化学 生物化学
背景情况:
- 希斯乙化调节了DNA的转录,复制和修复.
- 乙-共酶A (CoA) 对于基因素乙化至关重要.
- 酸盐脱酶复合体 (PDC) 主要以其在线粒体中的作用而闻名.
研究的目的:
- 为了研究核中pyruvate脱酶复合体 (PDC) 的存在和功能.
- 为了确定核PDC在乙-CoA合成和基因素乙化中的作用.
- 探索 mitochondria 和核之间 PDC 的动态调节和转移.
主要方法:
- 对哺乳动物细胞核中PDC亚单元局部化的分析.
- 在孤立的功能核中,核PDC的淘汰.
- 测量 de novo 乙-CoA 合成和 ヒ斯乙化.
- 细胞周期分析和S阶段标记物表达的评估.
主要成果:
- 线粒体PDC的所有子单元都存在于哺乳动物细胞核中并具有功能.
- 核PDC倒置降低了de novo乙-CoA合成和核心组织素乙化.
- 核PDC水平表现出细胞周期依赖性,并随着血清,EGF或线粒体应激增加,与线粒体PDC降低相关.
- 抑制核PDC会损害对G1-S阶段过渡和S阶段标志物表达至关重要的基因组乙化.
结论:
- 线粒体PDC转移到核中,提供核乙-CoA合成的来源.
- 核PDC在基因素乙化和表观遗传调节中起着至关重要的作用.
- 通过表观遗传修饰,PDC的动态穿支持细胞循环的进展.
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