细胞骨紧张阻碍了Hippo通过Ajuba-Warts复合体发出的信号
Cordelia Rauskolb1, Shuguo Sun1, Gongping Sun1
1Howard Hughes Medical Institute, Waksman Institute and Department of Molecular Biology and Biochemistry, Rutgers The State University of New Jersey, Piscataway NJ 08854 USA.
Cell
|July 5, 2014
概括
细胞骨张力通过调节河马路径直接影响器官生长. 增加的张力通过约克转录因子促进了Drosophila翅膀的生长,而减少的张力则减少了它.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
背景情况:
- 机械力量与器官生长有关,但潜在的分子机制仍然不清楚.
- 需要在细胞骨张力和体内生长调节之间的直接联系.
研究的目的:
- 阐明连接细胞骨张力与器官生长调节的分子机制.
- 为了确定参与这种机械转导过程的关键蛋白质和途径.
主要方法:
- 使用Drosophila melanogaster作为一个模型生物.
- 研究了细胞骨张力,肌活性和Hippo通路组件 (Yorkie, Warts, Ajuba LIM蛋白Jub) 的作用.
- 使用遗传和生化方法检查了粘附结和相互作用的蛋白质定位.
主要成果:
- 增加的细胞骨张力促进了Drosophila翅膀的生长,而减少的张力则抑制了它.
- 细胞骨张力调节约克 (Yki) 转录因子的活动,这是河马通路的下游效应因子.
- 阿朱巴LIM蛋白Jub作为一个关键的调解者,将肌活性和细胞骨张力与Hippo通路内的的激酶活性联系起来.
- 朱布在粘附连接处的局部化和与α-catenin的关联是紧张依赖的,促进了的招募.
结论:
- 描述了一种新的机制,其中细胞骨张力通过Jub.调节Hippo通路活动.
- 提供了关于机械力如何影响器官生长的分子理解 in vivo.
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