相关实验视频
Updated: Jun 16, 2026

14:49
Associated Chromosome Trap for Identifying Long-range DNA Interactions
Published on: April 23, 2011
双激活蛋白的DNA特异性是由家庭主体识别螺旋残留9决定的
Cell
|June 30, 1989
概括
在Bicoid家庭主体蛋白中改变单个氨基酸将其DNA结合特异性切换. 这一发现表明,识别螺旋的残留9决定了Bicoid和相关的家庭主体蛋白中的DNA特异性.
科学领域:
- 发展生物学 发展生物学
- 分子遗传学 分子遗传学
- 蛋白质工程是指蛋白质工程.
背景情况:
- 双基因对于Drosophila胚胎的前部结构形成至关重要.
- 含有家庭主体的比科伊德蛋白质可能会调节巴 (hb) 基因的转录.
- 已被证明与DNA结合的双融合蛋白可以刺激基因表达.
研究的目的:
- 为了研究由Bicoid家庭主体进行DNA识别.
- 为了确定负责Bicoid的DNA结合特异性的关键残留物.
- 为了探索改变Bicoid的DNA结合性能的潜力.
主要方法:
- 利用基于酵母的基因激活试验来研究DNA识别.
- 采用位点定向的突变发生,以改变比科因主体的特定氨基酸.
- 分析了野生型和突变的Bicoid蛋白质的DNA结合特异性.
主要成果:
- 在识别螺旋的第9位单个氨基酸替代改变了Bicoid的DNA特异性.
- 突变的Bicoid蛋白质识别了DNA部位,通常与其他家庭主体蛋白质结合,如Ultrabithorax和fushi tarazu.
- 证明残留物9对于确定DNA序列识别至关重要.
结论:
- 在Bicoid和Antennapedia类家庭主体蛋白中的DNA特异性是由识别螺旋的残留9决定的.
- 这种残留物可以被修改为切换Bicoid的DNA结合偏好.
- 研究结果提供了关于胚胎发育期间转录因子特异性的分子机制的见解.
相关概念视频
DNA as a Genetic Template
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
DNA-only Transposons
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
The donor site from where the transposon is excised is either degraded or...
Single-Strand DNA Binding Proteins
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
Restriction Enzymes
Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
The DNA Helix
Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...
DNA as a Genetic Template
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...

