奶酪皮社区为微生物多样性的in situ和in vitro研究提供了可处理的系统
Benjamin E Wolfe1, Julie E Button1, Marcela Santarelli1
1FAS Center for Systems Biology, Harvard University, Cambridge, MA 02138, USA.
Cell
|July 19, 2014
概括
奶酪皮作为可处理的微生物群落,揭示可复制的组装模式和相互作用. 这种模型系统允许在受控环境中研究微生物多样性机制.
科学领域:
- 微生物学 微生物学
- 社区生态学 社区生态学
- 食品科学 食品科学 食品科学
背景情况:
- 了解微生物社区的组合和功能对于将观察模式与机械解释相结合至关重要.
- 需要模型系统来实验研究微生物多样性和相互作用.
研究的目的:
- 建立奶酪皮作为一个可处理的模型微生物社区.
- 在现场描述微生物多样性模式,并开发一个用于社区重建的体外系统.
主要方法:
- 来自10个国家的137个奶酪皮社区的测序.
- 在现场描述微生物多样性和时间采样.
- 开发一个用于社区重建和相互作用分析的体外系统.
主要成果:
- 在各种不同的奶酪皮上确定了24种主要的,可培养的细菌和真菌属.
- 观察到可复制的社区类型和聚集模式,独立于地理来源.
- 在一个简化的体外系统中成功地回顾了现场社区动态,揭示了广泛的相互作用.
结论:
- 奶酪皮为研究微生物社区的组合和功能提供了一个实验性可处理的系统.
- 可复制的微生物社区结构和动态可以在体外建立和研究.
- 该模型有助于识别驱动微生物社区聚集和生态相互作用的机制.
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