增强器循环在发育过程中看起来稳定,并与暂停的聚合酶相关
Yad Ghavi-Helm1, Felix A Klein2, Tibor Pakozdi2
1European Molecular Biology Laboratory, Genome Biology Unit, D-69117 Heidelberg, Germany.
Nature
|July 22, 2014
概括
发育增强剂在基因激活之前形成稳定的三维循环. 这些预先形成的增强剂-促进剂接触,在物种中保存,可能通过释放暂停的RNA聚合酶来触发转录.
科学领域:
- 基因组学就是基因组学.
- 发展生物学 发展生物学
- 基因法规 基因法规
背景情况:
- 发育增强剂对于基因调节,进化和疾病至关重要.
- 了解增强剂-促进剂相互作用及其在胚胎发生过程中的动态是有限的.
- 现有的模型显示出特定位置的相互作用动态 (例如,β-环球蛋白与HoxD).
研究的目的:
- 以高分辨率绘制Drosophila胚胎发生过程中增强器三维接触的地图.
- 研究增强剂-促进剂相互作用的动态和特性,跨发育阶段和组织环境.
- 了解转录是如何从这些相互作用中启动的.
主要方法:
- 在Drosophila胚胎中的增强器三维接触的高分辨率映射.
- 跨越两个发育阶段和组织背景的分析.
- 对相互作用模式和与转录机制的关联进行比较分析.
主要成果:
- 长距离增强剂-增强剂和增强剂-促进剂相互作用在紧的Drosophila基因组中很普遍.
- 大多数相互作用在发育和组织类型中都是稳定的,在基因激活之前发生.
- 相互作用经常涉及增强剂与具有相似表达模式的多个促进剂接触,并与暂停的RNA聚合酶相关.
结论:
- 增强器接触的一般拓在和人类之间得到保留.
- 转录启动可能通过通过暂停的RNA聚合酶释放的预制增强剂-促进剂循环发生.
- 这些发现提供了关于在发育过程中基因调节的基本机制的见解.
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