在RNA中以基媒介形成5-基甲基细胞因子
Lijuan Fu1, Candace R Guerrero, Na Zhong
1Environmental Toxicology Graduate Program and ‡Department of Chemistry, University of California , Riverside, California 92521, United States.
Journal of the American Chemical Society
|July 30, 2014
概括
十一种转位 (Tet) 酶在DNA中氧化5-甲基细胞因,以进行表观遗传调节. 研究人员发现,Tet酶还在RNA中产生5-基甲基cytidine (5-hmrC),这表明了RNA生物学中的新角色.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 在RNA生物学,RNA生物学.
- 分子生物学分子生物学
背景情况:
- 十个十一个转位 (Tet) 酶已知可以氧化DNA中的5-甲基细胞素,这是表观遗传调节的关键过程.
- 除了DNA修饰之外,Tet酶的精确功能和点仍在探索中.
研究的目的:
- 调查TET酶是否对RNA表现出活性.
- 为了确定5-基甲基cytidine (5-hmrC) 是否由Tet酶在RNA中产生.
- 探索RNA氧化在哺乳动物细胞中的存在和潜在作用.
主要方法:
- 使用Tet酶和RNA基质进行体外酶分析.
- 在人体细胞中使用Tet酶催化域和全长Tet3的基于细胞的实验.
- 在细胞和组织RNA中量化5-基甲基cytidine (5-hmrC) 水平.
主要成果:
- 在试验室中发现,tet酶可催化RNA中的5-基甲基 (5-hmrC) 的形成.
- 所有三种Tet酶的催化域和全长的Tet3诱导了人体细胞中的5-hmrC形成.
- 在哺乳动物细胞和组织的RNA中检测到显著的5-hmrC水平 (大约每5000个5-methylcytidine中的1个).
结论:
- 酶具有一种新的氧化RNA的活性.
- 酶在RNA中形成5-hmrC表明了潜在的新基因调节层.
- 这一发现表明RNA氧化参与了基本的生物过程.
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