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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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由Cas9内核酶识别PAM依赖的目标DNA的结构基础

Carolin Anders1, Ole Niewoehner1, Alessia Duerst1

  • 1Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.

Nature
|August 1, 2014
PubMed
概括

克里斯普尔-Cas9系统使用导向RNA在特定位置切割DNA. 这项研究揭示了Cas9与DNA结合的晶体结构,详细介绍了它如何识别PAM序列以进行精确的基因组编辑.

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