相关实验视频

Updated: Apr 26, 2026

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

在急性白血病中对Notch调节的长非编码RNA进行全基因组映射和表征

Thomas Trimarchi¹, Erhan Bilal², Panagiotis Ntziachristos¹

¹Howard Hughes Medical Institute, Laura and Isaac Perlmutter Cancer Center, and Helen L. and Martin S. Kimmel Center for Stem Cell Biology, NYU School of Medicine, 550 First Avenue, New York, NY 10016, USA; Department of Pathology, NYU School of Medicine, 550 First Avenue, New York, NY 10016, USA.

|August 2, 2014

概括

长非编码RNAs (lncRNAs) 是T细胞急性淋巴细胞白血病 (T-ALL) 中瘤性Notch1信号的下游目标. 一种特定的lncRNA,LUNAR1,通过增强IGF1R信号传递来驱动T-ALL的生长.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

痕信号在发育过程中至关重要,在人类癌症中经常发生变化.
异常的Notch1信号驱动T细胞急性淋巴细胞白血病 (T-ALL) 的发生.
作为T-ALL中Notch信号的下游目标的长非编码RNAs (lncRNAs) 的作用尚不清楚.

研究的目的:

研究 lncRNA 基因作为T-ALL.中的致癌性Notch1的下游标.
通过Notch1.1调节的新型T-ALL特异性lncRNAs的识别.
为了确定在T-ALL生长中的Notch调节lncRNAs的功能作用.

主要方法:

转录组概况的整合与染色体状态映射.
对Notch1/Rpbjκ直接转录标的分析.
在体外和体外功能测试以评估T-ALL生长.

主要成果:

发现了许多T-ALL特异性lncRNA基因.
通过Notch1/Rpbjκ复合体直接控制的lncRNAs的识别.
证明LUNAR1,一个Notch调节的lncRNA,通过调节IGF1RmRNA并维持IGF1信号,促进T-ALL增殖.

结论:

lncRNAs是T-ALL.中Notch信号通路的重要下游目标.
划分调节的lncRNAs,如LUNAR1,是T-ALL.中瘤原体状态的关键调节者.
针对 lncRNAs 可能为T-ALL提供新的治疗策略.

更多相关视频

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

Published on: December 4, 2018

相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

Notch Signaling Pathway

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...

Notch Signaling Pathway

Notch Signaling Pathway

The Nucleolus

The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...