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通过全身清除透明完整组织内的单细胞表型
Bin Yang1, Jennifer B Treweek1, Rajan P Kulkarni2
1Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Cell
|August 5, 2014
概括
新的组织清除技术使全器官成像成为可能,为了解细胞结构和功能创建详细的3D地图. 这些方法有助于在完整的器官中绘制正常和病理元素的高分辨率映射.
科学领域:
- 神经科学是一个神经科学.
- 生物技术是生物技术.
- 解剖成像 解剖成像 解剖成像
背景情况:
- 三维解剖和表型图对于理解跨尺度的结构功能关系至关重要.
- 由于缺乏全器官成像方法,存在重大知识差距.
- 目前的局限性阻碍了对细胞结构和器官内部连接的全面绘制.
研究的目的:
- 介绍全器官和全身组织清除和成像的新技术.
- 为了使生物结构的高分辨率,高内容映射和表型化.
- 为了解决缺乏创建完整器官详细3D解剖图的方法的问题.
主要方法:
- 被动清晰技术 (PACT) 用于被动组织清除和完整器官的免疫染色.
- 折射率匹配溶液 (RIMS) 作为用于成像厚组织的安装介质.
- perfusion-assisted agent release in situ (PARS) 用于全身清除和免疫标记. 通过输液辅助的物质在现场释放,用于全身清除和免疫标记.
主要成果:
- PACT,RIMS和PARS使整个器官和身体具有宏分子透性和光学透明性.
- 方法与内源光,免疫组织化学和动物的RNA单分子FISH兼容.
- 证明了具有长期存储能力的细胞和亚细胞分辨率成像.
结论:
- 开发的技术克服了全器官成像方面的局限性,使得详细的3D解剖绘图成为可能.
- 这些方法促进了完整的生物系统中的正常和病理元素的高分辨率,高含量表型化.
- 通过增强的成像能力,PACT,RIMS和PARS协议为促进生物和医学研究提供了一个多功能工具包.
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