石油的生物降解. 石油中的水滴是微生物生命的微生物息地
Rainer U Meckenstock1, Frederick von Netzer2, Christine Stumpp2
1Institute of Groundwater Ecology, Helmholtz Zentrum München, 85764 Neuherberg, Germany. rainer.meckenstock@uni-due.de.
概括
皮奇湖内的微小水滴中的微生物.
科学领域:
- 地质化学 地质化学
- 微生物学 微生物学
- 石油科学 石油科学 石油科学
背景情况:
- 碳化合物的生物降解通常发生在油水过渡区.
- 油库的质量受到无氧微生物降解的影响.
- 特立尼达和多巴哥的皮奇湖是世界上最大的青湖.
研究的目的:
- 为了调查微生物活动和碳化合物的生物降解在油中被困的水滴.
- 描述这些独特环境中的微生物群落及其代谢过程.
- 评估石油在常规油水过渡区之外的生物降解潜力.
主要方法:
- 单个滴滴微生物组的Pyrotag测序.
- 核磁共振 (NMR) 和里叶变换离子循环子共振质谱 (FT-ICR-MS) 用于代谢物分析.
- 分析水的盐度和稳定的同位素.
主要成果:
- 在微小的 (13微升) 水滴中发现了代谢活跃,复杂的甲原微生物群落.
- 这些社区积极将石油降解成各种各样的代谢物.
- 高盐度和水稳定的同位素表明这些水滴的来源在地下深处.
结论:
- 碳化合物的微生物降解可以发生在被油困的水滴中,而不仅仅是在油水过渡区.
- 这些水滴的高含水量和大表面积代表了石油生物降解的显著,低估的潜力.
- 这些发现对了解油库质量和地下微生物过程有影响.
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