具有延长因子4的晶体结构与一个顺时针的核糖体结合在一起
Matthieu G Gagnon1, Jinzhong Lin2, David Bulkley3
1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8114, USA. Howard Hughes Medical Institute, Yale University, New Haven, CT 06520-8114, USA.
概括
延长因子4 (EF4/LepA),一个翻译因子,在Thermus thermophilus核糖体上进行结构分析. 它与P位点tRNA和核糖体重塑的相互作用表明它在蛋白质合成调节中的作用.
科学领域:
- 分子生物学分子生物学
- 结构生物学 结构生物学
- 生物化学 生物化学
背景情况:
- 延长因子4 (EF4/LepA) 是一种参与蛋白质合成的保存型GTPase.
- EF4/LepA影响tRNA转位,并与核糖体复合体相互作用.
研究的目的:
- 阐明EF4/LepA功能的结构机制.
- 为了确定EF4/LepA与核糖体和tRNA的相互作用.
主要方法:
- 使用X射线结晶学来获得结构.
- 该结构以2.9安格斯特罗姆分辨率确定.
主要成果:
- 确定了EF4-GDP与P位点tRNA结合Thermus thermophilus核糖体的结晶结构.
- EF4的C端域与P位点中的基tRNA相互作用.
- 核糖体表现出一种不寻常的状状态,其解码中心被改造.
结论:
- 该结构提供了关于EF4/LepA作为逆转位酶或核糖体隔离因子的作用的见解.
- EF4/LepA与核糖体的相互作用影响蛋白质合成延长.
更多相关视频
相关概念视频
Directing Proteins to the Rough Endoplasmic Reticulum
12.0K
The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
12.0K
Ribosomes
59.9K
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
59.9K
Ribosomes
9.2K
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
9.2K
Ribosomes
3.3K
3.3K
Ribosomes
16.0K
16.0K
Ribosomal RNA Synthesis
12.3K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
12.3K


