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相关概念视频

Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Replication in Eukaryotes01:29

Replication in Eukaryotes

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In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
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相关实验视频

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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
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复制和重组之间的永恒但及时的联系.

Valérie Borde1, Michael Lichten2

  • 1CNRS UMR3664, Institut Curie, Centre de Recherche, Paris 75248, France.

Cell
|August 16, 2014
PubMed
概括

DNA双链断裂的形成,对于中介性重组至关重要,与DNA复制协调. 这种协调取决于TIM-TIPIN复合体在复制分叉中招募一个关键激酶.

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 细胞生物学 细胞生物学

背景情况:

  • 介质重组开始于DNA双链断裂 (DSB).
  • 形成DSB的时间与 DNA 复制在变化过程中的时间协调在一起.
  • 了解这种协调是介质变化和基因组稳定性的关键.

研究的目的:

  • 阐明协调DSB形成与复制的分子机制.
  • 为了确定参与招募关键酶到复制叉中的蛋白质.

主要方法:

  • 在酵母模型中利用遗传和生物化学方法.
  • 研究了TIM-TIPIN复合体在招募Dbf4-依赖激酶 (DDK) 中的作用.
  • 分析了DSB形成和复制叉的进展.

主要成果:

  • 证明保存的TIM-TIPIN复合体将Dbf4-依赖激酶 (DDK) 引入复制分叉.
  • 表明这种招募对于DSB形成与复制的时间协调至关重要.
  • 确定了连接复制和重组启动的保存机制.

结论:

  • TIM-TIPIN复合体作为支架,将复制机器与DSB形成机器连接起来.

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  • 这种机制确保DSB在S阶段的适当时间发生.
  • 这些发现表明,这种调节途径也可能控制其他与复制相关的过程.