沙门氏菌在宿主组织中的表型变异延迟了通过抗微生物化疗的根除
Beatrice Claudi1, Petra Spröte2, Anna Chirkova1
1Focal Area Infection Biology, Biozentrum, University of Basel, 4056 Basel, Switzerland.
Cell
|August 16, 2014
概括
大多数抗生素治疗对于持久性细菌是失败的,导致慢性感染. 这项研究表明,宿主环境使细菌生长多样化,影响疾病的进展和治疗的有效性.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 细菌病原体的产生
背景情况:
- 抗生素治疗经常无法根除细菌的持续存在,导致复发和慢性感染.
- 虽然体外研究确定了细菌持久性和抗菌素耐受性的机制,但体外的意义尚不清楚.
研究的目的:
- 调查细菌表型变化的体内相关性及其对抗生素治疗疗效的影响.
- 了解宿主环境如何影响细菌生理学和疾病进展.
主要方法:
- 利用光增长率报告器监测宿主组织中的沙门氏菌生理学.
- 在单细胞水平上跟踪了抗生素化疗期间的沙门氏菌生长和存活率.
主要成果:
- 在宿主组织中观察到沙门氏菌的广泛的表型异质性,包括生长缓慢和生长快的子集.
- 发现抗生素杀死与细菌分裂率相关,非分裂细菌的生存率最高,但很少见.
- 确定大多数幸存的细菌起源于适度生长,部分耐受性种群.
结论:
- 宿主组织诱导细菌群体显著的生理多样化.
- 细菌的表型变异,特别是适度生长的持久性,在疾病进展中起着至关重要的作用,并限制了抗生素治疗的有效性.
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