一个超分子宿主-客人载体系统,用于增长因子,采用V(H) H片段
Jordi Cabanas-Danés1, Emilie Dooms Rodrigues, Ellie Landman
1Molecular Nanofabrication Group, MESA+ Institute for Nanotechnology, Faculty of Science and Technology, University of Twente , P.O. Box 217, 7500 AE Enschede, Netherlands.
Journal of the American Chemical Society
|August 26, 2014
概括
这项研究提出了一种新型的超分子策略,使用His6标记的抗体可逆地固定生长因子,从而实现受控递送,并证明其在组织工程应用中的细胞分化中的生物活性.
科学领域:
- 生物材料科学 生物材料科学
- 超分子化学 超分子化学
- 蛋白质工程是指蛋白质工程.
背景情况:
- 增长因子的受控组合对于组织再生至关重要.
- 现有的方法往往缺乏可逆性和时间控制.
- 超分子化学为精确的分子组装提供直角相互作用.
研究的目的:
- 开发一种可逆的超分子增长因子固定化策略.
- 为组装利用直角主机-客户和金属亲和相互作用.
- 为了证明固定生长因子的生物活性.
主要方法:
- 针对hBMP6.6的His6标记单域抗体 (VHH) 的重组表达.
- 通过阿达曼提尔 (Ad) 和N-酸 (NTA) - 希斯提丁 (His) 相互作用,在β-环氧化 (βCD) 表面上组装.
- 使用微接触打印 (μCP),表面等离子体共振 (SPR) 和微尺度热泳 (MST) 的表征.
- 在细胞分化实验中通过性酸酶 (ALP) 检测评估生物活性.
主要成果:
- 在βCD表面上成功组装了VHH抗体的均和可逆层.
- 人类骨形态遗传蛋白-6 (hBMP6) 被有效地固定在功能化的表面上.
- 动不动的hBMP6表现出生物活性,促进原生细胞早期的骨质分化.
- 超分子策略提供了亲和力,可逆性和对生长因子交付的时间控制.
结论:
- 开发的超分子策略使得增长因子的精确和可逆组合成为可能.
- 这种方法促进了生长因子的功能传递,促进了特定的细胞反应.
- 这种方法对再生医学和生物材料的应用具有前景.
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