相关实验视频
Updated: Apr 25, 2026

10:52
Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems
Published on: October 14, 2025
1.4K
通过转录依赖的CRISPR-Cas向对温菌体的有条件耐受性
Gregory W Goldberg1, Wenyan Jiang1, David Bikard2
1Laboratory of Bacteriology, The Rockefeller University, New York, New York 10065, USA.
Nature
|September 2, 2014
概括
斯塔菲洛科克斯 (Staphylococcus epidermidis) 的CRISPR-Cas系统可以防止菌体感染,同时可以容忍有益的温带菌体. 这种有条件的耐受性,通过转录依赖的向实现,保持细菌的遗传稳定性.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- Prokaryotic 免疫系统,如 CRISPR-Cas,可以区分病原体和自我/共生体.
- 克里斯普尔-卡斯系统使用RNA引导的核酶来切割外来DNA,主要针对菌体基因组.
- 温和菌体可以整合到细菌基因组中,提供健康益处,但对非歧视性的CRISPR-Cas免疫构成挑战.
研究的目的:
- 为了研究Staphylococcus epidermidisCRISPR-Cas系统的向机制.
- 为了确定CRISPR-Cas系统是否能够区分性和性菌体感染.
- 探索转录依赖向对细菌适应性免疫的影响.
主要方法:
- 分析了斯塔菲洛科克斯表皮菌的CRISPR-Cas系统与温带菌体的相互作用.
- 调查转录在CRISPR-Cas向特异性中的作用.
- 评估CRISPR-Cas活性对菌体溶解和溶液周期的影响.
主要成果:
- 斯塔菲洛科克斯表皮菌的CRISPR-Cas系统有效地防止了菌体感染.
- 该系统对经过溶解酶化的温带菌体表现出耐受性.
- 有条件的耐受性是由一个依赖转录的DNA向机制介导的.
- 导向在诱导导体流动周期时被重新激活.
结论:
- 克里斯普尔-卡斯系统在其准机制中表现出功能上的分歧.
- 转录依赖的向允许对有益的 lysogenic 菌体产生耐受性,从而保持遗传稳定性.
- 这项研究将"对非自我耐受"的概念扩展到 prokaryotic 适应性免疫.
相关概念视频
CRISPR and crRNAs
14.5K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
14.5K
The Antiviral System of Bacteria and Archaea: CRISPR
1.0K
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
1.0K
CRISPR/Cas9 Genome Editing
3.1K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
3.1K
CRISPR
46.3K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
46.3K
CRISPR
13.8K
13.8K
Conservative Site-specific Recombination and Phase Variation
5.7K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
5.7K

