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完整的结构和表达在高亲和性IgE受体转染细胞中的表达

U Blank¹, C Ra, L Miller

¹Section on Chemical Immunology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Bethesda, Maryland 20892.

|January 12, 1989

概括

研究人员克隆了高亲和性免疫球蛋白E受体 (Fc epsilon RI) 的玛子单元,这对过敏反应至关重要. 所有子单元的共同表达使受体功能成为可能,为研究人类Fc epsilon RI相互作用铺平了道路.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
过敏研究研究过敏

背景情况:

高亲和性免疫球蛋白E受体 (Fc epsilon RI) 是过敏反应的关键,它调解着从巨细胞和基细胞中释放的介质.
Fc epsilon RI是动物中的四重复合体 (α,β,gamma2),对于IgE结合和信号传导至关重要.
之前的研究分离了α和β子单元cDNA,但未能达到IgE结合表达.

研究的目的:

克隆编码Fc epsilon RI的玛子单元的互补DNA (cDNA).
为Fc epsilon RI四聚合物 (αβ-二) 提出一个结构模型.
为了在转染细胞中实现Fc epsilon RI的功能表达.

主要方法:

补充DNA (cDNA) 克隆Fc epsilon RI玛子单元.
在COS 7细胞与编码alpha,beta和gamma子单元的cDNA的同时感染.
在Fc epsilon RIαββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββ

主要成果:

成功克隆了Fc epsilon RI马子单元cDNA.
动物Fc epsilon RI在COS 7细胞上的表达只有在所有三个亚单元cDNAs的共同传染时才能实现.
提出了αβ-γ2四聚合物的结构模型,解释了受体特征.

结论:

玛子单位对于Fc epsilon RI的功能表达至关重要.
所有Fc epsilon RI子单元的共同表达是受体组合和功能所必需的.
这项工作使人类Fc epsilon RI的研究和针对过敏疾病的向抑制剂的开发成为可能.

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