一个用于细胞增殖和分化的翻译调节的双重程序
Hila Gingold1, Disa Tehler2, Nanna R Christoffersen2
1Department of Molecular Genetics, The Weizmann Institute of Science, Rehovot 76100, Israel.
Cell
|September 13, 2014
概括
两个不同的转移RNA (tRNA) 子集调节细胞增殖和分化. 这些tRNA子集与基因表达协调,这表明细胞自主和多细胞功能的不同的转录程序.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 遗传学 是一个遗传学.
背景情况:
- 甲动物细胞在增殖和分化之间面临着一个基本的选择.
- 转移RNA (tRNA) 对于蛋白质合成至关重要,并且可以影响基因表达.
- 不同的细胞状态可能涉及tRNA和基因表达的独特调节机制.
研究的目的:
- 研究tRNA池和细胞状态之间的关系 (增殖与分化).
- 在与细胞自主功能和多细胞性相关的基因中识别不同的编码子使用模式.
- 在这些细胞过程中探索tRNA供应和基因转录之间的协调.
主要方法:
- 在各种细胞类型中测量tRNA池.
- 在与细胞自主功能和多细胞性相关的基因中对子使用的分析.
- 检查tRNA基因及其点基因附近的基因修饰.
主要成果:
- 确定了两个不同的tRNA子集:一个诱导在增殖细胞中,另一个诱导在分化细胞中.
- 发现细胞自主基因和多细胞性基因表现出不同的密码.
- 观察到tRNA基因及其相应的基因附近的基因修饰的协调变化,表明转录调节.
- 增殖诱导的tRNA与细胞自主基因中丰富的编码匹配;分化诱导的tRNA与多细胞性基因中丰富的编码匹配.
结论:
- 存在两个不同的翻译程序,在扩散和差异化期间运行.
- 转录程序协调tRNA供应与基因表达模式所决定的需求.
- 这种协调表明,一个监管机制将细胞命运决定与转化控制联系起来.
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