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在小鼠参考群体中的线粒体活动的多层遗传和奥米克解剖

Yibo Wu¹, Evan G Williams², Sébastien Dubuis¹

¹Department of Biology, Institute of Molecular Systems Biology, Eidgenössische Technische Hochschule Zürich (ETHZ), Zurich 8093, Switzerland.

|September 13, 2014

概括

遗传学和饮食显著影响复杂的特征. 这项研究整合了多种omics数据,以确定代谢表型的关键调节者,如Dhtkd1

科学领域:

基因组学和系统生物学
代谢学和蛋白质学

背景情况:

了解基因型与环境的相互作用对于复杂的表型至关重要.
BXD小鼠基因参考群体为此类研究提供了一个强大的模型.

研究的目的:

研究基因型和饮食如何影响分子形状和代谢表型.
为了确定复杂的代谢特征的遗传位置和分子调节器.

主要方法:

在两个饮食中的40个BXD小鼠菌株中量化了转录组,肝蛋白和代谢组.
利用向蛋白质组学和基因映射 (QTL分析).

主要成果:

发现了许多与代谢表型相关的转录,蛋白质和代谢物QTLs.
确定Dhtkd1作为2-氨基酸酸的关键调节剂,影响葡萄糖和糖尿病状态.
在物种中表现为可变但仍保留的线粒体展开蛋白质反应 (UPR(mt)).

结论:

综合的多学科方法对于剖析复杂的代谢表型是有价值的.
遗传和环境因素在代谢健康中起着重要作用.
Dhtkd1和UPR (mt) 是理解新陈代谢调节的重要目标.

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Animal Mitochondrial Genetics

Animal Mitochondrial Genetics

Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...