细胞质丁氨酸是膜有机细胞的负终端导向电机
T A Schroer1, E R Steuer, M P Sheetz
1Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110.
Cell
|March 24, 1989
概括
细胞质二烯酸驱动着沿微管子向减数端的有机细胞运输. 为了使这种运动蛋白有效地在运动中发挥作用,需要额外的细胞分裂因子.
科学领域:
- 细胞生物学 细胞生物学
- 分子运动功能分子运动功能
- 细胞骨的动力学
背景情况:
- 基于微管的运输对于细胞内组织至关重要.
- 细胞质丁氨酸是一种运动蛋白质,与器官细胞运动有关.
研究的目的:
- 调查细胞质二烯在有机体运输中的特定作用.
- 确定对细胞质二烯介导的细胞移动性的要求.
主要方法:
- 使用胚胎纤维细胞组件进行复制的体外检测.
- 采用瓦纳酸介导的紫外线光分裂来使细胞质二烯失活.
- 使用净化成分和细胞醇,评估了微管上有机细胞的运动.
主要成果:
- 高速细胞质诱导减去微管上终端导向的有机体运动.
- 细胞质体dynein的无活化消除了90%以上的这种运动性.
- 恢复纯化的细胞质丁氨酸到不活跃的细胞质,恢复了细胞的运动性.
结论:
- 细胞质丁氨酸是减小终端导向器官运输的主要驱动器.
- 额外的细胞结合因子对于细胞质丁氨酸来调节器官运动是必不可少的.
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