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Updated: Apr 23, 2026

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通过多价值N-酸载体复合体对被标记的蛋白质进行活细胞向

Ralph Wieneke1, Noemi Labòria, Malini Rajan

  • 1Institute of Biochemistry, Goethe-University Frankfurt , Max-von-Laue-Str. 9, D-60438 Frankfurt am Main, Germany.

Journal of the American Chemical Society
|September 20, 2014
PubMed
概括

我们开发了一种新方法,用于快速和特定的蛋白质标记在活细胞使用细胞透化剂复合体. 这种技术能够在细胞内实时跟踪用histidine标记的蛋白质,并且具有很高的准确性.

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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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