细胞保护性,ABC triblock聚合物基热反应性水凝,具有ROS触发的降解和药物释放
Mukesh K Gupta1, John R Martin, Thomas A Werfel
1Biomedical Engineering, Vanderbilt University , Nashville, Tennessee 37235, United States.
Journal of the American Chemical Society
|September 26, 2014
概括
研究人员从一个triblock聚合物 (PPS-b-PDMA-b-PNIPAAM) 开发了一种新型的可注射水凝,该聚合物在体温下形成稳定的凝. 这种先进的材料提供了可控的药物释放和增强的细胞保护,克服了传统水凝的局限性.
科学领域:
- 聚合物化学 聚合物化学
- 材料科学 材料科学 材料科学
- 生物医学工程 生物医学工程
背景情况:
- 传统的聚N-异烯胺 (PNIPAAM) 水凝存在诸如协同和缺乏可降解性等局限性.
- 需要可注射的,对刺激有反应的水凝,具有用于药物输送和组织工程的增强功能.
研究的目的:
- 为了合成和表征一种新的ABCtriblock共聚合物,聚[(烯硫化物) -块-(N,N-二甲基甲胺) -块-(N-异甲胺) ] (PPS-b-PDMA-b-PNIPAAM).
- 评估这种聚合物的潜力,以形成物理交叉链接的水凝,具有用于药物输送和细胞封装的可调性特性.
- 为了研究反应性氧物种 (ROS) 引发的降解和开发的水凝的药物释放能力.
主要方法:
- 用于聚合物合成,采用了阳离子和可逆添加碎片化链转移 (RAFT) 聚合.
- 通过改变聚合物度和温度来研究菌形成和随后的水凝.
- 风湿学测量被用来量化水凝的机械性能.
- 在体外和体内研究评估了药物释放动力学,水凝降解,细胞相容性和细胞保护作用.
主要成果:
- 合成的PPS-b-PDMA-b-PNIPAAM聚合物在环境温度下自组装成,并在生理温度 (37°C) 上形成稳定的水凝.
- 水凝的机械性能 (储存模量) 随着聚合物度的增加而增加.
- 水凝在反应性氧物种 (ROS) 的反应中显示出尼罗河红色的受控释放,并表现出ROS介导的降解.
- 实验室研究证实了细胞兼容性和细胞保护性,而体内研究显示药物持续释放14天.
结论:
- PPS-b-PDMA-b-PNIPAAM形成可注射,细胞保护性水凝,具有可调节的特性和对刺激有反应的药物释放.
- 这种新的水凝系统克服了传统PNIPAAM水凝的关键局限性,显示了生物医学应用的巨大潜力.
- 该材料适用于细胞封装和输送,在体内提供持续的药物释放.
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