短的细胞质序列作为保留信号,用于内膜网膜中的跨膜蛋白质
T Nilsson1, M Jackson, P A Peterson
1Department of Immunology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Cell
|August 25, 1989
概括
这种腺病毒E3/19K糖蛋白.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 病毒学 病毒学
背景情况:
- 腺病毒E3/19K糖蛋白是一种内细胞网膜 (ER) 居民.
- 了解蛋白质定位信号对于细胞生物学至关重要.
研究的目的:
- 为了确定特定的氨基酸序列,负责腺病毒E3/19K糖蛋白的ER保留.
- 为了确定这个序列是否可以赋予其他蛋白质ER居住性.
主要方法:
- 对E3/19K细胞质尾巴的删除分析.
- 将已识别的残留物移植到其他膜结合蛋白质上.
- 在内源性ER蛋白中分析ER保留信号.
主要成果:
- 细胞质尾巴E3/19K的C端六种氨基酸对于ER保留是必要的和足够的.
- 这种基因在转移到其他蛋白质时可以赋予ER居住.
- 没有单一的氨基酸是唯一负责的;图案的极端C端位置是关键的.
- 在内源性ER蛋白UDP-glucuronosyltransferase中发现了类似的保留信号.
结论:
- 简短的线性C端序列作为跨膜蛋白的ER保留信号.
- 这种机制在病毒和内源性ER居住蛋白中保持.
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