生物燃料.生物燃料. 改变的固醇成分使酵母具有耐热性
Luis Caspeta1, Yun Chen1, Payam Ghiaci2
1Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, SE-41296 Gothenburg, Sweden.
概括
研究人员通过调整它以在高温 (≥40°C) 中生长来增强生物燃料生产的酵母. 这涉及到基因变化,特别是固醇生物合成,提高了乙醇发酵效率.
科学领域:
- 生物技术是生物技术.
- 微生物学 微生物学
- 生物燃料生产 生物燃料生产
背景情况:
- 乙醇生物燃料的生产依赖于酵母发酵.
- 高温 (≥40°C) 提高工艺效率,降低成本.
- 目前的酵母菌株在高温下表现出不良的生长和发酵.
研究的目的:
- 开发具有更好的耐热性酵母菌株,用于高效的乙醇生产.
- 确定基因和代谢适应,使酵母在40°C以上的温度下生长.
主要方法:
- 适应性实验室进化 (ALE) 用于选择耐热酵母.
- 进行了全基因组测序,基因表达和代谢流动分析.
- 进行了固醇成分分析.
主要成果:
- ALE产生了能够在40°C以上的温度下生长和生产乙醇的酵母菌株.
- 在C-5固醇脱酶中的关键突变改变了固醇成分,从ergosterol变成fecosterol.
- 观察到醇生物合成基因的表达增加和染色体III重组.
结论:
- 酵母对高温的适应涉及到显著的固醇成分变化.
- 固醇的产生和改变的固醇生物合成对于耐热性至关重要.
- ALE对于工业生物燃料应用的工程酵母是有效的.
更多相关视频
10:10Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
Published on: September 20, 2016
14.0K
09:10Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass
Published on: June 24, 2016
23.8K
相关概念视频
Biofuels
105
The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...
105
Bioreactor Controls-III
67
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
67
Biosynthesis of Lipids
945
Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis...
945
Microbial Fermentation
1.7K
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
1.7K
Fates of Pyruvate
8.9K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.9K
Microbes in Food Production
383
Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
383
