两种不同的胺基域介导人体内皮细胞在体外分化成毛细血管状结构的过程
D S Grant1, K Tashiro, B Segui-Real
1Laboratory of Developmental Biology and Anomalies, National Institute of Dental Research, Bethesda, Maryland 20892.
Cell
|September 8, 1989
概括
内皮细胞在Matrigel上形成管状结构,这是由拉米尼开始的过程. 拉米林中的特定序列触发细胞附着和细胞与细胞相互作用,对于毛细血管形成至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 生物化学 生物化学
- 血管生物学 血管生物学
背景情况:
- 内皮细胞 (ECs) 在培养中分化缓慢.
- 基底膜蛋白质凝Matrigel促进了快速EC对齐和管道形成.
研究的目的:
- 为了阐明由拉米林诱导的内皮管形成的多步骤过程.
- 为了确定特定的拉米林序列和参与EC分化和毛细血管形态发生的受体.
主要方法:
- 在Matrigel上培养微血管和大血管内皮细胞.
- 在细胞培养实验中利用拉米因及其合成YIGSR.
- 研究整合素受体在EC附着和相互作用中的参与.
主要成果:
- 拉米宁通过多步骤的过程诱导内皮管的形成.
- 拉米因的A链中的RGD序列通过整合素受体介导EC附着.
- 拉米因B1链中的YIGSR位点促进细胞与细胞的相互作用和管道组装.
- 合成YIGSR单独可以诱导单个EC形成毛细血管状的环与光度.
结论:
- 拉米宁是内皮管形成的关键诱导剂.
- 不同的胺基域及其相应的细胞受体协调EC附着和细胞与细胞相互作用.
- YIGSR具有理解和操纵毛细血管形成的潜力.
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