一种 γ-氨基酸,有利于α/γ-酸中的 12/10 螺旋二次结构
Michael W Giuliano1, Stacy J Maynard, Aaron M Almeida
1Department of Chemistry, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.
Journal of the American Chemical Society
|October 11, 2014
概括
这项研究引入了一种新型的循环氨基酸,APCH,它在酸中强制执行独特的12/10螺旋结构,并交替替键方向. 这种独特的形状偏好不同于其他受约束的玛氨基酸.
科学领域:
- 类化学 类化学
- 结构生物学 结构生物学
- 有机合成 有机合成
背景情况:
- 传统的在螺旋体中表现出均的H-结合方向性.
- 异体和非自然可以形成带有交替H键方向的螺旋体.
研究的目的:
- 评估一种新型循环性玛氨基酸 (1R,2R,3S) - 2 - 2 - - 1 - 氨基) - 循环赫素碳酸 (APCH) 的结构偏好.
- 为了研究二级结构,强制APCH残留在骨中的二级结构.
主要方法:
- 立体选择性合成N受保护的APCH.
- 核磁共振 (NMR) 光谱学.核磁共振 (NMR) 光谱学.
- 一个X射线晶体学.
主要成果:
- APCH强烈强制执行一个α/gamma-peptide 12/10螺旋二次结构.
- 这种螺旋结构的特点是沿骨干交替的H键方向性.
- 与其他周期性玛氨基酸相比,APCH具有明显的构造倾向.
结论:
- 新型循环玛氨基酸APCH具有独特的形状偏好.
- APCH稳定了螺旋结构与交替的H键方向性.
- 这一发现扩大了用于设计新结构的氨基酸谱.
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