细胞形状和分裂方向的相互作用促进发育表皮质的强大形态发生
Fengzhu Xiong1, Wenzhe Ma1, Tom W Hiscock1
1Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.
Cell
|October 11, 2014
概括
斑马鱼胚胎上皮的发育是由细胞分裂方向和机械合形成的. 这项研究揭示了一种可进化的逻辑,用于强大的细胞水平调节组织形状.
科学领域:
- 发育生物学是发展生物学.
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
背景情况:
- 皮质细胞在发育过程中采取各种形状,对组织功能至关重要.
- 了解组织几何学,细胞分裂和机制之间的相互作用是破译上皮质形态发生的关键.
研究的目的:
- 分析组织几何,细胞分裂和机制如何相互作用,形成斑马鱼胚胎假定包裹层 (pre-EVL).
- 研究细胞形状分布和细胞分裂方向之间的反循环.
主要方法:
- 结合了理论建模,定量成像和斑马鱼胚胎中的实验性扰动.
- 开发了细胞形状-分裂方向反循环的综合数学模型.
主要成果:
- 确定EVL前的平整是通过从几何约束下的差异性定向划分的细胞数量变化来调节的.
- 证明细胞分裂模式是由细胞形状分布决定的,受机械合的影响.
- 在体内证实,细胞形状对组织表面积,细胞体积和细胞数量的变化具有坚固性.
结论:
- 提出了一个可进化的设计逻辑,使组织发育能够在细胞水平上进行强有力的控制.
- 表明将细胞形状和分裂方向联系在一起的参数是上皮质多样性的来源.
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