通过母细胞和女细胞的中心球放大在多细胞中有所不同
Adel Al Jord1, Anne-Iris Lemaître1, Nathalie Delgehyr1
11] Ecole Normale Supérieure, Institut de Biologie de l'ENS, IBENS, F-75005 Paris, France [2] Inserm, U1024, F-75005 Paris, France [3] CNRS, UMR 8197, F-75005 Paris, France.
Nature
|October 14, 2014
概括
在多细胞中,中心的重复并不是 de novo. 相反,新的中心体起源于祖细胞中心体内的母中心体,挑战了以前对细胞分裂的理解.
科学领域:
- 细胞生物学 细胞生物学
- 中心体生物学 中心体生物学
- 的生成 (Ciliogenesis) 是一种
背景情况:
- 中心细胞由母细胞和女细胞组成,在循环细胞中通常会对称地复制,保持细胞分裂的恒常性.
- 多细胞表现出明显的中心放大过程,形成多个中心为运动,其起源往往归因于de novo形成围绕deuterosomes.
- 在多胞胎发生过程中,中枢细胞的确切起源和双胞胎细胞的作用在很大程度上是未知的.
研究的目的:
- 为了阐明多细胞在多细胞的多生成过程中形成的众多中心体的起源.
- 调查现有的中枢细胞和子细胞在新中枢细胞形成中的作用.
- 要确定在这种情况下的中心极重复是否遵循原型的半保守模型或不同的机制.
主要方法:
- 使用实时成像技术实时观察心心形成.
- 相关超分辨率光和电子显微镜用于高分辨率结构分析.
- 这些方法允许追踪中心极的起源及其与双胞胎细胞的关联.
主要成果:
- 在多细胞中,所有新形成的中间体都源于先前存在的原始细胞中心体,通过重复的百分离体播种.
- 只有中心体内的子中心体才有助于双胞胎体的形成.
- 这种女儿中心衍生途径占这些细胞中最终中心群体的90%以上.
结论:
- 在多细胞中,中心球的形成不是 de novo,而是源于中心体,特别是涉及子中心球.
- 这一发现揭示了中心极对放大贡献的意想不到的不对称性.
- 了解这种机制为与乳毛相关的疾病和癌症和小头症中的病理性中心点放大提供了新的见解.
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